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A b s t r a c t Despite the popularity of antigen-retrieval techniques, the precise molecular mechanism underlying the process remains enigmatic. We examined the molecular features underlying the loss of immunoreactivity following formalin fixation, with subsequent recovery by antigen retrieval. To do this, we first created a molecular model using short peptides that mimic the antibody-binding site of common clinical protein targets. The advantage of this model is that we know the amino aciddoi:10.1309/brn7ctx1e84nwwpl pmid:14983931 fatcat:tk7omghqbvexvlmten3ypcuvsm