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Multipotent stem cells maintain the structure and function of the mammary gland throughout its development and respond to the physiological demands associated with pregnancy and lactation. The ability of mammary stem cells to maintain themselves as well as to give rise to differentiated progeny is not only affected by soluble factors but has increasingly become linked to mechanical cues including the elastic modulus of the extracellular matrix (ECM). Here we describe a protocol for determiningdoi:10.1007/7651_2013_31 pmid:24014310 fatcat:pxcky3xoyjbv7lnlht6y5wg7de