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The Pfam database is a widely used resource for classifying protein sequences into families and domains. Since Pfam was last described in this journal, over 350 new families have been added in Pfam 33.1 and numerous improvements have been made to existing entries. To facilitate research on COVID-19, we have revised the Pfam entries that cover the SARS-CoV-2 proteome, and built new entries for regions that were not covered by Pfam. We have reintroduced Pfam-B which provides an automaticallydoi:10.1093/nar/gkaa913 pmid:33125078 pmcid:PMC7779014 fatcat:qhulduagfrg2jj7n25n5sppaue