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Inducible Selectable Marker Genes to Improve Aspergillus fumigatus Genetic Manipulation
2021
Journal of Fungi
The hygromycin B phosphotransferase gene from Escherichia coli and the pyrithiamine resistance gene from Aspergillus oryzae are two dominant selectable marker genes widely used to genetically manipulate several fungal species. Despite the recent development of CRISPR/Cas9 and marker-free systems, in vitro molecular tools to study Aspergillus fumigatus, which is a saprophytic fungus causing life-threatening diseases in immunocompromised hosts, still rely extensively on the use of dominant
doi:10.3390/jof7070506
pmid:34202756
pmcid:PMC8305790
fatcat:52n4qqkefvaojoqqm23gkfxsme