Water droplet-in-oil digestion method for single-cell proteomics [article]

Takeshi Masuda, Yuma Inamori, Arisu Furukawa, Kazuki Momosaki, Chih-Hsiang Chang, Daiki Kobayashi, Hiroto Ohguchi, Yawara Kawano, Shingo Ito, Norie Araki, Shao-En Ong, Sumio Ohtsuki
2021 bioRxiv   pre-print
Recent advances in single-cell proteomics highlight the promise of sensitive analyses in limited cell populations. However, technical challenges remain for sample recovery, throughput, and versatility. Here, we first report a water droplet-in-oil digestion (WinO) method based on carboxyl-coated beads and phase transfer surfactants for proteomic analysis using limited sample amounts. This method was developed to minimize the contact area between the sample solution and the container to reduce
more » ... loss of proteins and peptides by adsorption. This method increased protein and peptide recovery 10-fold as well as the number of quantified transmembrane proteins compared to an in-solution digestion (ISD) method. The proteome profiles obtained from 100 cells using the WinO method highly correlated with those from 10000 cells using the ISD method. We successfully applied the WinO method to single-cell proteomics and quantified 462 proteins. Using the WinO method, samples can be easily prepared in a multi-well plate, making it a widely applicable and suitable method for single-cell proteomics.
doi:10.1101/2021.12.13.472378 fatcat:bpuypk43ybe5li4bn5ybmv2oiu