An Epstein-Barr virus transformation-associated membrane protein interacts with src family tyrosine kinases

A L Burkhardt, J B Bolen, E Kieff, R Longnecker
1992 Journal of Virology  
In latently infected growth-transformed human lymphocytes, Epstein-Barr virus (EBV) encodes two integral plasma membrane proteins: LMP1, which constitutively induces B-lymphocyte activation and intercellular adhesion, and LMP2A, which associates with LMP1 and is a tyrosine kinase substrate. We now demonstrate that LMP2A associates with src family protein tyrosine kinases, particularly lyn kinase, in nonionic detergent extracts of transfected B lymphoma cells or in extracts of EBV-transformed B
more » ... ymphocytes. The LMP2A and tyrosine kinase association is stable in nonionic detergents and includes a 70-kDa cell protein which is also an in vitro or in vivo kinase substrate. This LMP2A association with B-lymphocyte src family tyrosine kinases is likely to be an important pathway in EBV's effects on cell growth. J. VIROL.
doi:10.1128/jvi.66.8.5161-5167.1992 fatcat:2q4m36pknvhlrmped7z7bunuh4