Phytochemical analysis and hepatoprotective activity of Raphanus sativus var. sativus in Sprague-Dawley rats

Heshu Sulaiman Rahman, Kashan Alaalddin Bayz, Ridha Hassan Hussein, Azad Ismael Abdalla, Hemn Hassan Othman, Kawa Mohammad Amin, Rasedee Abdullah
2020 Tropical Journal of Pharmaceutical Research  
Purpose: To determine the phenolic and flavonoid contents of R. sativus rhizome ethanol extract and the hepatoprotective effect of the extract in rats. Methods: Folin–Ciocalteau and aluminum chloride colorimetric tests were used to determine the contents of phenols and flavonoids in the R. sativus extract. Male Sprague-Dawley rats induced with CCl4 to develop hepatotoxicity were treated orally with R. sativus extract for 4 weeks. The antioxidant and anti-inflammatory effects of the extract on
more » ... e liver were determined by evaluating the concentration of oxidative analytes, serum liver enzymes and lipids, and hepatic histopathology and cytochrome P450 2E1 expression. Results: R. sativus extract significantly (p < 0.05) reduced the hepatotoxic effect of CCl4 via its antioxidant activities and protection of liver tissues from oxidative damage. Conclusion: The hepatoprotective effects of R. sativus rhizome ethanol extract are attributed to its highphenolic and flavonoid contents. Keywords: R. savitus rhizome, Phenols, Flavonoid contents, antioxidant, Hepatoprotective
doi:10.4314/tjpr.v19i8.25 fatcat:5dmewi7u4bcubbr4xzrw5kftim