Litter sampling is utilized as a noninvasive and practical method to determine broiler flock Salmonella status. The common methods include boot cover/sock (BC), drag swab (DS), or litter grab sampling (LG). Roller swabs are a new research method that can be used to sample litter without entering research pens. This study aimed to assess the use of roller swabs (RS) for Salmonella (S) detection in comparison to BC, DS, and LG. For Experiment 1, litter was sampled for two weeks following a

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... flock that was challenged at 6 days of age with 1 £ 10 7 cfu of a nalidixic acid resistant strain of Salmonella Enteritidis to establish a high litter prevalence of S. In Experiment 2, sampling occurred after a subsequent flock was raised on the same litter. In Experiment 1, S was detected by RS less frequently (81%) than DS (95%), BC (97%), and LG (98%; P < 0.0001). In Experiment 2, S detection using RS (23%) was similar to LG (17%), higher than DS (6%, P = 0.022), and lower than BC (55%, P < 0.0001). Although RS were a less sensitive sampling method when the litter prevalence of S was high, RS were equivalent to or better than LG and DS methods when S prevalence was low. The use of roller swabs allows for sampling of litter without entering each pen and has the potential to be utilized for Salmonella detection in research pen trials.