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Simultaneous detection of Epstein - Barr virus and Cytomegalovirus DNA in Mini-pooling of Whole blood Samples from Iraqi blood donors by Multiplex Real-Time PCR Assay
2020
Annals of Tropical Medicine and Public Health
A qualitative multiplex real-time PCR assay was used for simultaneously detection of Epstein-Barr virus (EBV), cytomegalovirus (CMV) DNA within blood donors by using 10 sample mini-pools (MP10) of whole blood from blood donors using a commercial multiplex PCR kit. The multiplex assay saved time and was cost effective through using blood mini-pools. A total of 1000 whole-blood specimens were collected from blood donors and pooled in 100 MP10 and were categorized to 50 pools after 6h from blood
doi:10.36295/asro.2020.231441
fatcat:74dqbx3dinemdhopbe7z6pfuku