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The Natural Phototransforming Photochrome Membrane Protein Bacteriorhodopsin From Purple Membranes of Halobacterium Halobacterium Halobium
Nanotechnology Research and Practice
This paper presents improved method for isolation of photochrome transmembraine protein bacteriorhodopsin (output 5 mg from 100 g of wet biomass) capable to transform light energy to electrochemical energy of generated protons H + and АТP. The protein was isolated from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight moleculardoi:10.13187/ejnr.2014.1.43 fatcat:zqbhi7vbqbcd5af6bbjcj4gcyu