Production of anti-complement antibody for development of anti-human globulin serum for blood banking use

Preeyanat Vongchan, Kedsarin Chantan
2015 Journal of Associated Medical Sciences  
The anliglobulin test is commonly used in serological laboratory, especially blood bank. Direct antiglobufin test is used to investigate the in vivo coating of red cells including hemolytic disease of the newborn (HDN), autoimmune hemolytic anemia and transfusion reactions. In another aspect, indirect antiglobulin test is used to detect alloantibodies in patient sera ,n yltro. Both direct and indirect antiglobulin tests are then linally observed by anti-human globulin (AHG) polyspecific reagent
more » ... which contains anti-human globulin and anti-complement. Anti-complement can be used not only in antiglobulin test but a variety ot immunological assays. As facilities in production of in-house antibodies are available in our laboratory, we therefore produced rabbit polyclonal antibodies specific to human complement(s) using zymozan as an inducer of complement activation pathway of AB serum from normal human. Hemagglutination of complement coated red blood cells was used to assay anti-complement in rabbit sera. We present polyclonal antibodies specific to complement C3b, C3d, C4b and C4d from our study. lt was also shown that the obtained antibodies has an inhibition effecl on complement induced sensitized red blood cell hemolysis. The anti-complement will be further on combine with anti human globulin to produce the working AHG reagenl. Moreover, it can be used as a tool in many immunological assays. Bull Msi Assoc Med Sci 2005; 3Er lE5-192.
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