Compromised liver mitochondrial function and complex activity in low feed efficient broilers are associated with higher oxidative stress and differential protein expression

M. Iqbal, N. R. Pumford, Z. X. Tang, K. Lassiter, C. Ojano-Dirain, T. Wing, M. Cooper, W. Bottje
2005 Poultry Science  
Variations in broiler growth and efficiency have been explained in part by differences in mitochondrial function and biochemistry in broilers. To further our knowledge in this regard, 2 experiments were carried out to determine the relationships of a) mitochondrial function and activities of various electron transport chain (ETC) complexes; b) production of H 2 O 2 , a reactive oxygen species (ROS), and its association with protein oxidation; and c) mitochondrial protein expression in liver of
more » ... single line male broilers with low or high feed efficiency (FE, n = 5 to 8 per group). Mitochondrial function and complex activities were measured polarographically and spectrophotometrically, respectively. H 2 O 2 was measured fluorimetrically, whereas oxidized protein (carbonyls) and specific mitochondrial proteins were analyzed using Western blots. Mitochondrial function (ETC coupling) and activities of ETC complexes (I, II, III, and IV)
doi:10.1093/ps/84.6.933 pmid:15971533 fatcat:huucrg5zjnfxbcl65f6qjhwyza