Sulfated polyanions block Chlamydia trachomatis infection of cervix-derived human epithelia

F R Zaretzky, R Pearce-Pratt, D M Phillips
1995 Infection and Immunity  
Using a cell line derived from the human cervix and a rapid fluorescence cytotoxicity assay, we have shown that Chlamydia trachomatis infection can be blocked by certain sulfated polysaccharides (carrageenan, pentosan polysulfate, fucoidan, and dextran sulfate) and glycosaminoglycans (heparin, heparan sulfate, and dermatan sulfate) but not by other glycosaminoglycans (chondroitin sulfate A or C, keratan sulfate, and hyaluronic acid). The most negatively charged molecules are the most effective
more » ... the most effective at blocking infection. Results of infection at 4؇C suggest that sulfated polyanions act by preventing the adherence of chlamydiae to target cells. These and additional blocking studies with enzymes suggest that a heparan sulfate-like glycosaminoglycan on the surface of elementary bodies is involved in the adherence of chlamydiae to target cells, probably through a nonspecific charge interaction or possibly a heparin-binding protein. We previously observed that the same sulfated polysaccharides inhibit transmission of human immunodeficiency virus in vitro and suggested that these compounds could be used in a vaginal formulation to inhibit infection by human immuno deficiency virus. The results of the present study suggest that the same type of formulation may inhibit sexual transmission of chlamydia. on May 7, 2020 by guest http://iai.asm.org/ Downloaded from a Effect of sulfated polysaccharides and GAGs on serovar E infection of ME180 cells at 37ЊC. IC 50 , 50% inhibitory concentration range.
doi:10.1128/iai.63.9.3520-3526.1995 fatcat:hqo5jcsom5e6dl6wmxffy3cejy