A Multi-Pronged Approach to Assessing Inflammatory Inhibitory Activity of a New Cyclic Alkaloid Compound from Root Bark of Ziziphus Nummularia (Aubrev.) [post]

Sarbani Dey Ray, Nirupam Das, Supratim Ray
2021 unpublished
[(16-methoxy-10-(3-methyl-butyl)-2-oxa-6, 9, 12-triaza-tricyclo [, 7] nonadeca-1(18), 13, 15 (19), 16-tetraene-8, 11-Dione], a putative cyclic alkaloid compound (IC) isolated from the root bark of Ziziphus nummularia, showed potential anti-inflammatory potential. Nitric oxide (NO), prostaglandin-E2 (PGE2), and tumour necrosis factor-alpha (TNF- α) levels were measured in vitro to assess IC's potential. ADME simulations and molecular docking of IC by TNF- α receptor were also performed.
more » ... The in vivo potentials of IC and ethanolic extract (EE) were investigated by assessing carrageenan-induced paw oedema and arachidonic acid/xylene-induced ear oedema. TNF-α inhibition was higher in IC than in others, with a maximal percent inhibition of 88.00 percent at 50.11 µM. IC generated hydrogen bonds with ASP 45 and GLN 47, according to in silico research. Carrageenan, xylene, and arachidonic acid-induced oedema were all significantly reduced by IC. As a result, IC may have clinical potential in the future treatment of inflammation.
doi:10.21203/rs.3.rs-1134126/v1 fatcat:lllzvhq4nzc4vngangwo2xi3zm