Online) An Open Access

Mansour Shokri, Mostafa Faghani
2016 Online International Journal Available at   unpublished
This study was performed for evaluation of second introns and third exon polymorphism of leptin and its effect on growth parameters by gene sequencing in Lori Bakhtiari ram lambs. For this reason a total of 86 Lori Bakhtiari sheep breeds were used. All lambs were identified at birth, and birth weights, as well as sex, birth type, and pedigree information was recorded. Polymerase chain reaction (PCR) products were subjected to single strand conformation polymorphism (SSCP) denaturation and
more » ... aturation and polyacrylamide gel electrophoresis. Result showed that there were no significant differences for S3201G/A and S3208G/T according to weight traits at the birth stage and 1-3,3-6,6-9 and 9-12 month. The relationship between weight traits with S3405A/T marker mutation showed that significant effects on weight at the birth stage (4.08±015 g.d) and body weight gain until one to three months (295.94±10.19 g.d) respectively. In conclusion we could confirm that potential usefulness of GH and LEP genes in marker assisted selection programs of sheep breeding.