Enhancing Our Understanding of Plant Cell-to-Cell Interactions Using Single-Cell Omics

Sandra Thibivilliers, Marc Libault
2021 Frontiers in Plant Science  
Plants are composed of cells that physically interact and constantly adapt to their environment. To reveal the contribution of each plant cells to the biology of the entire organism, their molecular, morphological, and physiological attributes must be quantified and analyzed in the context of the morphology of the plant organs. The emergence of single-cell/nucleus omics technologies now allows plant biologists to access different modalities of individual cells including their epigenome and
more » ... criptome to reveal the unique molecular properties of each cell composing the plant and their dynamic regulation during cell differentiation and in response to their environment. In this manuscript, we provide a perspective regarding the challenges and strategies to collect plant single-cell biological datasets and their analysis in the context of cellular interactions. As an example, we provide an analysis of the transcriptional regulation of the Arabidopsis genes controlling the differentiation of the root hair cells at the single-cell level. We also discuss the perspective of the use of spatial profiling to complement existing plant single-cell omics.
doi:10.3389/fpls.2021.696811 pmid:34421948 pmcid:PMC8375048 fatcat:2juyfg3nyrbv3ix44cztdwmpou