Sez-6 May Play an Important Role in Neurite Outgrowth through the PKCγ Signaling Pathways

Jing Zhang, Jun Zhang, Youjun Wu, Yang Zhou, Zhihua Shao, Xuan Kang, Lin Ma, Mengjie Li, Lihong Liu, Hongjun Shi
2011 Zeitschrift für Naturforschung C - A Journal of Biosciences  
Seizure-related gene 6 (sez-6) was originally identified in a study of pentylenetetrazoletreated cortical neurons. Further studies on the structure and expression pattern suggested that Sez-6 may play an important role in neuronal development and function. In the present study, PC12 cells were used as a model to investigate the role of Sez-6 in neurite outgrowth. After a period of NGF treatment, the expression of Sez-6 in PC12 cells was increased. When Sez-6 expression was suppressed by the
more » ... ppressed by the addition of an effective short hairpin RNA ( shRNA) plasmid, the neurite outgrowth was significantly inhibited. In addition, we detected the expression level of protein kinase Cγ (PKCγ), and found that the PKCγ protein level was reduced in the differentiated PC12 cells but increased in PC12 cells lacking Sez-6. Taken together, our results indicate that Sez-6 acts on the neurite outgrowth of PC12 cells likely through the PKCγ signaling pathways
doi:10.1515/znc-2011-11-1211 pmid:22351987 fatcat:dznetdoze5cz3hz44viioulrqi