A disulfide bond, formed by two oxidized cysteines, plays an important role in the protein folding and structure stability, and it may regulate protein functions. The disulfide connectivity prediction problem is to reveal the correct information of disulfide connectivity in the target protein. It is difficult because the number of possible patterns grows rapidly with respect to the number of cysteines. In this paper, we discover some rules to discriminate the patterns with high accuracy in

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... us methods. Then, we propose the pattern-wise and pairwise BKS (behavior knowledge space) methods to fuse multiple classifiers constructed by the SVM (support vector machine) methods. Furthermore, we combine the CSP (cysteine separation profile) method to form our hybrid method. The prediction accuracy of our hybrid method in SP39 dataset with 4-fold cross-validation is increased to 69.1%, which is better than the best previous result 65.9%.