The cecal microflora and short-chain fatty acid concentrations were determined in a 4-week trial with Wistar female rats (initial body weight ca. 50g) which were fed standard-or vitamin-free-purified diets containing galactosyllactose (GL). In rats fed the standard purified (SP) diet with a high dose (5%) of GL bifidobacteria, lactobacilli, and anaerobic Gram-positive cocci increased remarkably in number compared with diets containing lactose. Vitamin-free purified (VFP) diets, regardless of

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... amount (0, 2.5, and 5%) of GL added, resulted in marked increases of the above-mentioned bacteria. In the VFP diet with 5% GL, bifidobacteria and enterococci increased in number, but anaerobic Gram-positive cocci, Bacteroidaceae, and Enterobacteriaceae decreased compared with the 2.5% GL group. Staphylococci decreased in number in the VFP diets containing GL compared with the other groups. In rats fed the SP diets with GL, the total short-chain fatty, acetic, and propionic acids increased in concentration, and n-butyric, iso-butyric, and, iso-valeric acids were reduced. In rats fed VFP diets with or without GL, the concentrations of 1993年2月1日 受 付 *〒162東 京 都 新 宿 区 戸 山1-23-1 Toyama-cho , Shinjuku-ku, Tokyo162, Japan