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Xenobiotics transporter MRPl (Multidrug Resistance Protein 1) cloned from bovine-Comparison of its expression, structure and functions with human and mouse MRPl

Mem, Kinki School, University
2005 unpublished

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Previously, we cloned bovine MRP1 cDNA from cow's mammary gland, as a candidate for genes that make influence on quality of milk. In this paper, we investigated the tissue distribution of MRP 1 mRNA in bovine, and compared the amino acid sequence of bovine MRP1 with those of human and mouse MRPI. Northern blotting showed that the expression of bovine MRP1 mRNA occurs in a wide range of tissues, but little in liver and brain, as described in the case of human and mouse MRPl. Bovine MRP1 consists
more » ... of 1530 amino acids with 91 % and 87% identity with the human and mouse ortholog, respectively. The comparison of amino acid sequences and functions among bovine, human and mouse MRP 1 s revealed that the poor ability to confer resistance to doxorubicin of bovine MRP 1 is likely due to the fact that the amino acid in bovine MRP1 corresponding to Glu 1089 in human MRP1 is Gln1088 as in the mouse ortholog. Our results support the notion that this amino acid residue of MRP1 plays an important role in the activity of this transporter, especially in the ability to confer resistance to doxorubicin.
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Citation

Mem, Kinki School, University. "Xenobiotics transporter MRPl (Multidrug Resistance Protein 1) cloned from bovine-Comparison of its expression, structure and functions with human and mouse MRPl." (2005)