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Streptococcus pyogenes isolated from blood and urine samples obtained from a 78-year-old woman was tested for susceptibility, and fluoroquinolone resistance (minimum inhibitory concentration of levofloxacin, 16 mg/mL) was found. DNA amplification and sequencing revealed a serine 81 r tyrosine substitution in gyrA and 2 substitutions in parC: serine 79 rphenylalanine and alanine 121 rvaline. This is the second report of a clinical isolate of S. pyogenes with high-level fluoroquinolone resistance.doi:10.1086/345904 pmid:12539083 fatcat:yxavil3vqncr5ev6xj4tzt42oq