An efficient and optimized PCR method with high fidelity for site-directed mutagenesis

Q Liang, L Chen, A J Fulco
1995 Genome Research  
We have developed an efficient method for site-directed mutagenesis using two subsequential rounds of PCR. In this method, PCR conditions are optimized to favor high fidelity of PCR Methods and Applications 269 Receive free email alerts when new articles cite this article -sign up in the box at the http://genome.cshlp.org/subscriptions
doi:10.1101/gr.4.5.269 fatcat:4slkwjwaqrb2rl264to7rxtxcu