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Synthesis of protein by the obligate intracellular parasitic bacteria Chlamydia psittaci (6BC) and Chiamydia trachomatis (serovar L2) isolated from host cells (host-free chlaniydiae) was demonstrated for the first time. Incorporation of [35S]methionine and [35S]cysteine into trichloroacetic acid-precipitable material by reticulate bodies of chlamydiae persisted for 2 h and was dependent upon a exogenous source of ATP, an ATP-regenerating system, and potassium or sodium ions. Magnesium ions anddoi:10.1128/jb.162.3.938-942.1985 fatcat:vfawue4rcnehjbst43rcwnwtly