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This paper describes a fully automated assay on the Serono SRI™ for the measurement of cortisol in serum, heparinised plasma and urine. The assay incorporates a specific polyclonal antibody to cortisol and cortisol conjugated to alkaline phosphatase as a label. Following immunoincubation, bound and free labelled cortisol are separated by magnetic sedimentation of the antibody complex. Phenolphthalein is liberated by the enzymatic hydrolysis of the substrate (phenolphthalein monophosphate) anddoi:10.1515/cclm.19126.96.36.1991 fatcat:pti7oxqzprderowgn5t2a2ypgy