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The first meiotic cell division (meiotic maturation) of the dictyate stage of mouse oocytes, removed from the follicle, resumes spontaneously in vitro. This study indicates that estramustine phosphate reversibly blocks meiotic maturation by inhibiting germinal vesicle breakdown. Oocyte cryostat sections were stained with anti-tubulin and two antibodies (anti-MAP1 : JA2 and 5051) which decorated the metaphase spindle during meiotic maturation. It was found that (1) estramustine phosphatedoi:10.1051/rnd:19880211 fatcat:au3j2gqhxfhlpnvyzvdex2mhuu