Objectives Human phospholipid scramblase 1 (hPLSCR1) is a multifunctional protein so it is vital to understand the regulation of its expression and transcription factors play critical role in regulating any gene.Results The transcription factor (TF) prediction tool ConSite identified one S-SOX5 binding site on the promoter region of hPLSCR1. Luciferase assays determined the increase in hPLSCR1 promoter activity with the increase in S-SOX5 concentration in a dose dependent manner. The deletion

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... d the site-directed mutagenesis constructs exhibited a decrease in the hPLSCR1 promoter activity as compared to control. Also, transcriptional regulation of hPLSCR1 by S-SOX5 was checked by western blotting using S-SOX5 and hPLSCR1 specific antibodies. Further, Chromatin Immunoprecipitation (ChIP) assay confirmed the in-vivo interaction of S-SOX5 with hPLSCR1 promoter. Conclusions The expression of hPLSCR1 in HEK293T cells was shown to be upregulated by one S-SOX5 binding site present on the hPLSCR1 promoter suggesting that S-SOX5 as the potent regulator of hPLSCR1 expression.