The genus Ballota L. (BL) belongs to the Labiateae family spread throughout the world. Plants of this genus possess anti oxidant, anti cancer, anti microbial and anti inflammatory properties. This study aimed to investigate the antioxidant activities of extract taken from aerial parts of Ballota platyloma Rech. f. using percolation, ultrasonic and polyphenol fraction methods. Materials and Methods: In this experimental study, aerial parts of Ballota platyloma were collected from Veresk

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... an, Iran). Dried aerial parts of Ballota platyloma were extracted by percolation, ultrasonic and polyphenol fraction. The antioxidant activity of extracts was investigated using different methods: 1, 1-diphenyl-2-picryl hydrazyl (DPPH), iron ion chelating activity and reducing power. Data were analyzed using ANOVA and Duncan test at a significant level of P<0.05. Results: The total phenolic and total flavonoid contents were in order of polyphenol> ultrasonic > percolation, respectively. The IC 50 for polyphenol, percolation and ultrasonic extracts in DPPH radical-scavenging activity were 7.52±1.07, 6.64±0.87 and 2.58±0.06, respectively. The IC 50 for iron chelating ability were in order of polyphenol < ultrasonic < percolation. Polyphenol fraction showed better reducing power than the ultrasonic and percolation extracts (P<0.001) which was comparable to that of Vitamin C (P<0.05). Conclusion: The results showed that polyphenol fraction was rich in total phenolic and total flavonoid contents. Polyphenol fraction showed the best activity in reducing power but ultrasonic extracts was the most potent one in DPPH radical-scavenging activity. Thus, antioxidant activity may be attributed to the presence of phenols and flavonoids in the extracts.