Inhibitory Proteins in the Newcastle Disease Virus-Induced Suppression of Cell Protein Synthesis

D. P. Bolognesi, D. E. Wilson
1966 Journal of Bacteriology  
BOLOGNESI, D. P. (Rensselaer Polytechnic Institute, Troy, N.Y.), AND D. E. WILSON. Inhibitory proteins in the Newcastle disease virus-induced suppression of cell protein synthesis. J. Bacteriol. 91:1896Bacteriol. 91: -1901Bacteriol. 91: . 1966.-Infection by Newcastle disease virus brings about a rapid and marked inhibition of cell protein synthesis (CPS) in chick embryo fibroblast monolayers. The block to CPS is initiated about 5 hr after infection, and by 9 hr about 85% of the host protein
more » ... he host protein synthesis is shut off. Azauridine (3 mg/ml), a ribonucleic acid (RNA) synthesis inhibitor, prevents the virus-induced inhibition of CPS when added at the time of infection; but it does not prevent the inhibition when added at 3 hr after infection. When puromycin (60 ug/ml), a protein synthesis inhibitor, was added at 3.5 hr after infection, viral RNA was synthesized in normal amounts, but the virus-induced inhibition of CPo was prevented. Actinomycin D added at the time of infection does not, however, prevent the virus-induced inhibition of CPS. The results of these experiments indicate that proteins synthesized during Newcastle disease virus replication are responsible for the inhibition of host-cell protein synthesis. The synthesis of these inhibitory proteins depends on the prior synthesis of viral RNA. 1896 on May 4, 2020 by guest
doi:10.1128/jb.91.5.1896-1901.1966 fatcat:tis5q7us4jf7hlq57tjxcjky3e