AbstractThere is clinical demand for simple and contact-free diagnosis techniques in medical practice. This study shows that the infrared absorptions of volatile metabolites can be used to distinguish between the air around Pseudomonas aeruginosa, Acinetobacter baumannii, other bacteria, and normal room air. Gas samples were collected from the air surrounding single and mixed laboratory cultures, and preliminary data on human breath samples are also presented. The infrared spectra of a variety

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... f gasses are measured with a high resolution of 0.5 cm-1 to obtain information about the wavenumber position of the key bands. Hence, it is not necessary to specify the molecular species of biomarkers. This work also shows that discrimination rates can be improved by observing additional infrared absorptions caused by different characteristic volatile molecules. The significance of this work is that the specific wavenumber positions of the key bands that allow the application of infrared lasers are provided. As a result, it is considered that Pseudomonas aeruginosa and Acinetobacter baumannii can be monitored more sensitively and easily. With further research and development, this simple approach could be used in future applications to identify infections in healthcare settings.