Competitive Inhibition of Human Immunodeficiency Virus Type-1 Protease by the Gag-Pol Transframe Protein

Christina Paulus, Susanne Hellebrand, Uwe Tessmer, Hans Wolf, Hans-Georg Kräusslich, Ralf Wagner
1999 Journal of Biological Chemistry  
The human immunodeficiency virus type-1 (HIV-1) transframe protein p6* is located between the structural and enzymatic domains of the Gag-Pol polyprotein, flanked by the nucleocapsid (NC) and the protease (PR) domain at its amino and carboxyl termini, respectively. Here, we report that recombinant highly purified HIV-1 p6* specifically inhibits mature HIV-1 PR activity. Kinetic analyses and cross-linking experiments revealed a competitive mechanism for PR inhibition by p6*. We further
more » ... e that the four carboxyl-terminal residues of p6* are essential but not sufficient for p6*mediated inhibition of PR activity. Based on these results, we suggest a role of the transframe protein p6* in regulating HIV-1 PR activity during viral replication.
doi:10.1074/jbc.274.31.21539 pmid:10419458 fatcat:as3wzhsomzdixl656abgjwc6qy