Evaluation of platelet aggregation in platelet concentrates (PCs): storage implications

Teresinha J. C. Neiva, Marcos J. Machado, Monica Hoehn, Elizabeth M. Hermes, Cidônia L. Vituri, Jovino S. Ferreira, Elbio A. D'Amico
2004 Revista Brasileira de Hematologia e Hemoterapia  
The use of hemo-derivatives is nowadays a fundamentally important therapeutic modality in the exercise of medicine. Among the various hemo-components employed, we have the platelet concentrate (PC), indicated in cases of hemorrhagic disturbances. We previously showed that platelet function in blood donors is reduced in their screening phase and after the separation process of PCs. Currently, we are providing evidence for the existence of biochemical and functional changes in PC preparations
more » ... ed for three days at temperatures of 20 ± 2 °C. Platelet concentrates from 40 healthy donors, collected in CPD anticoagulant and PL-146 polyvinylchloride containers, were examined in order to determine the pH value, pCO 2 , pO 2 and lactate concentrations. In addition, the aggregation of platelets with thrombin and collagen were examined to evaluate platelet function. A pH increase from 7.07 ± 0.04 to 7.36 ± 0.07 (p < 0.01) was observed. The pCO 2 concentration decreased progressively from 69.2 ± 7.7 mmHg to 28.8 ± 6.2 mmHg (p < 0.001) during the storage period. In contrast, pO 2 value increase from 103.4 ± 30.6 to 152.3 ± 24.6 mmHg (p < 0.001) was evidenced during the 48 hours of storage. The lactate concentration increased from 17.97 ± 5.2 to 57.21 ± 5.7 mg/dL (p < 0.001). Platelet aggregation using 0.25 U/mL-thrombin and 2.0µg/mL-collagen showed significant hypofunction from 61.8 ± 2.7% to 24.8 ± 9.8% and 62.7 ± 5.0 to 33.4 ± 6.2 (p < 0.001), respectively. We concluded that the evaluated biochemical parameters and the platelet function changed significantly when the platelets were kept under routine storage conditions.
doi:10.1590/s1516-84842004000100015 fatcat:2afzxynma5akxcv3aibvzhgl7e