Protein Array Technology

Konrad B??ssow, Zolt??n Konthur, Angelika Lueking, Hans Lehrach, Gerald Walter
2001 American Journal of Pharmacogenomics  
The generation of protein chips requires much more efforts than DNA microchips. While DNA is DNA and a variety of different DNA molecules behave stable in a hybridisation experiment, proteins are much more difficult to produce and to handle. Outside of a narrow range of environmental conditions, proteins will denature, lose their three-dimensional structure and a lot of their specificity and activity. The chapter describes the pitfalls and challenges in Protein Microarray technology to produce
more » ... ative and functional proteins and store them in a native and special environment for every single spot on an array, making applications like antibody profiling and serum screening possible not only on denatured arrays but also on native protein arrays.
doi:10.2165/00129785-200101010-00005 pmid:12173313 fatcat:5hkarcluufb4xirx5wo4kwcmxu