Correction for Sharma et al., Dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is regulated by an RNA-protein scaffold complex

2011 Proceedings of the National Academy of Sciences of the United States of America  
A C IB: NFAT1 IB: IQGAP1 IB: IQGAP2 IB: CaM 670 kDa 158 kDa 52 54 56 58 60 62 64 66 68 IB: CK1 ε IB: CnA B 100 bp Fr. 54-60 Fr. 87-93 Load H2O Fr. 74-80 NFAT1: High protein Low protein + -- Fig. 1. NFAT1 coelutes with IQGAP and NRON in resting T cell lysates by size-exclusion chromatography. Hypotonic lysates from (A and B) HA-NFAT1 Jurkat T cells or (C) primary murine CD8 + T cells were fractionated on a Superdex 200 size-exclusion column. (A and C) Individual fractions were analyzed by
more » ... E and Western blotting for NFAT1, IQGAP1, IQGAP2, calmodulin (CaM), casein kinase epsilon (CK1ε) and calcineurin A (CnA). Column void volume (V) is indicated. (B) Pooled column fractions were analyzed for NRON sequences by RT-PCR.
doi:10.1073/pnas.1114486108 fatcat:qb3ksmrribhwlm5a5krkyww6wq