A greenhouse soil infested with an obligate nematode parasitic bacterium, Pasteuria penetrans, was used to test the effect of temperatures on the endospore attachment to root-knot nematode, Meloidogyne arenaria, juveniles (J2). Freshly hatched J2s were inoculated to the soil in petri dish and incubated under different temperatures of 20℃, 25℃, 30℃, and 35℃ for 7 days. The endospore attachment rates were 100% in all the temperatures, while the number of endospores attached per J2 was highest in

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... 5℃ with 28.3 endospores/J2 followed by 20.2, 18.6, and 13.6 in 30℃, 20℃, and 35℃, respectively. When the soil was pre-treated under different temperatures before the J2 inoculation, the endospore attachment rates significantly decreased from 60% in room temperature to 25.0, 31.7, 8.3, 5.0, and 0% after the soil incubation in -30℃, 4℃, 40℃, 50℃, and 100℃ for 10 days, respectively. The endospore numbers attached per J2 were 3.5, 4.3, 1, 1, and 0 when the soil was pre-treated in -30℃, 4℃, 40℃, 50℃, and 100℃, respectively, which were lower than 5.3/J2 of room temperature treated soil. The P. penetrans isolate in the soil showed nematode species-specific endospore attachment characteristics with 100% attachment rate only on M. arenaria J2s while the rates were 0% on M. hapla and M. incognita J2s. , 0개로 적 었다. P. penetrans 세균의 내생포자를 뿌리혹선충 종별로 J2에 부착 시험한 결과 땅콩뿌리혹선충에서는 100%였으나 당근뿌리혹선충(M. hapla)과 고구마뿌리혹선충(M. incognita)에서는 모두 0%로 본 균주는 뿌리혹선충 종에 대해 기주선호성을 가진 것으로 나타났다.