Attachment of Pasteuria penetrans Endospores to Meloidogyne spp. Juveniles Affected by Temperatures and the Nematode species
선충기생세균(Pasteuria penetrans) 내생포자의 뿌리혹선충(Meloidogyne spp.) 유충 부착에 대한 온도와 선충종의 영향

Myoung Rae Cho, Hyung Hwan Kim, Taek Joon Kang, Seung Joon Ahn, Chang Yul Yang, Sung Wook Jeon
2013 Korean journal of applied entomology  
A greenhouse soil infested with an obligate nematode parasitic bacterium, Pasteuria penetrans, was used to test the effect of temperatures on the endospore attachment to root-knot nematode, Meloidogyne arenaria, juveniles (J2). Freshly hatched J2s were inoculated to the soil in petri dish and incubated under different temperatures of 20℃, 25℃, 30℃, and 35℃ for 7 days. The endospore attachment rates were 100% in all the temperatures, while the number of endospores attached per J2 was highest in
more » ... 5℃ with 28.3 endospores/J2 followed by 20.2, 18.6, and 13.6 in 30℃, 20℃, and 35℃, respectively. When the soil was pre-treated under different temperatures before the J2 inoculation, the endospore attachment rates significantly decreased from 60% in room temperature to 25.0, 31.7, 8.3, 5.0, and 0% after the soil incubation in -30℃, 4℃, 40℃, 50℃, and 100℃ for 10 days, respectively. The endospore numbers attached per J2 were 3.5, 4.3, 1, 1, and 0 when the soil was pre-treated in -30℃, 4℃, 40℃, 50℃, and 100℃, respectively, which were lower than 5.3/J2 of room temperature treated soil. The P. penetrans isolate in the soil showed nematode species-specific endospore attachment characteristics with 100% attachment rate only on M. arenaria J2s while the rates were 0% on M. hapla and M. incognita J2s. , 0개로 적 었다. P. penetrans 세균의 내생포자를 뿌리혹선충 종별로 J2에 부착 시험한 결과 땅콩뿌리혹선충에서는 100%였으나 당근뿌리혹선충(M. hapla)과 고구마뿌리혹선충(M. incognita)에서는 모두 0%로 본 균주는 뿌리혹선충 종에 대해 기주선호성을 가진 것으로 나타났다.
doi:10.5656/ksae.2013.03.0.012 fatcat:gbv74ufw3fgyhcvay3opgzclfi