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One-Day Construction Of Multiplex Arrays to Harness Natural CRISPR Systems
[article]
2020
bioRxiv
pre-print
CRISPR-Cas systems are prokaryotic immune systems that have proliferated widely not only in bacteria and archaea, but also much more recently, in human biological research and applications. Much work to date has utilized synthetic sgRNAs along with the CRISPR nuclease Cas9, but the discovery of array-processing nucleases now allows the use of more compact, natural CRISPR arrays in heterologous hosts, in addition to organisms with endogenous systems. Unfortunately, the construction of multiplex
doi:10.1101/2020.03.06.981316
fatcat:hy2jzfjzyfdezagtgcxxxeaz6m