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Purpose: To improve the production of a novel exopolysaccharide (EPS) by Bacillus mucilaginosus CGMCC5766. Methods: The culture medium for production of EPS was optimized using statistical experiment design. Sucrose, CaCO3 and K2HPO4 were found to be the key factors based on the results obtained from Plackett-Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and then the optimum concentration was further investigateddoi:10.4314/tjpr.v12i5.8 fatcat:wsy6keelfnb5thslpy6hr3btym