Arabidopsis Tubulin Folding Cofactor B Interacts with α-Tubulin In Vivo

Pankaj Dhonukshe, Bastiaan O.R. Bargmann, Theodorus W.J. Gadella
2006 Plant and Cell Physiology  
Microtubule biogenesis requires ab tubulin dimers that are generated from a and b tubulin following posttranslational modification by several tubulin folding cofactors (TFCs). Here we report the isolation and characterization of Arabidopsis TFCB (AtTFCB). AtTFCB is expressed in all organs of Arabidopsis. The subcellular localization of AtTFCB is mainly cytosolic. AtTFCB-overexpressing cells have fewer microtubules compared with the controls. Multimode fluorescence resonance energy transfer
more » ... ) microscopy reveals a direct physical interaction of AtTFCB with a tubulin in living plant cells. We conclude that AtTFCB interacts with a tubulin in vivo and its overexpression reduces the number of microtubules.
doi:10.1093/pcp/pcl001 pmid:16928693 fatcat:m2orh4aqqjcqbiz55zq32f6bqy