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Identification of small RNAs in Mycobacterium smegmatis using heterologous Hfq
2012
RNA: A publication of the RNA Society
Gene regulation by small RNAs (sRNAs) has been extensively studied in various bacteria. However, the presence and roles of sRNAs in mycobacteria remain largely unclear. Immunoprecipitation of RNA chaperone Hfq to enrich for sRNAs is one of the effective methods to isolate sRNAs. However, the lack of an identified mycobacterial hfq restricts the feasibility of this approach. We developed a novel method that takes advantage of the conserved inherent sRNAs-binding capability of heterologous Hfq
doi:10.1261/rna.034116.112
pmid:23169799
pmcid:PMC3527728
fatcat:vui7abrejvepdpz3ugnx6qjwra