Week long effects of applied stress on the production of artemisinin and precursors in Artemisia annua L. plants

Anders Kjaer, Heng Yin, Francel Verstappen, Harro Bouwmeester, Elise Ivarsen, Xavier Fretté, Lars Christensen, Porskjaer, Kai Grevsen, Martin Jensen, A Kjaer, F Verstappen (+10 others)
2015 Journal of Plant Biology Research   unpublished
The purpose was to examine the week long stress effect on full grown plants of Artemisia annua (A. annua) with attention to the content of artemisinin (AN) and its immediate precursors in young leaves. In two separate experiments, clonally propagated plants were stress treated either once or weekly for five weeks by sandblasting or spraying with salicylic acid, chitosan oligosaccharide, H2O2 or NaCl. Contents of AN and AN related compounds (AN-c) were analysed in leaf samples from the upper
more » ... from the upper part of plants using triple-quadrupole UPLC-MRM-MS. Results showed that concentrations of the quantified AN and AN-c were statistically unchanged in response to multiple stress treatments, whereas plants responded to singularly applied stress treatments by a response wave of temporarily lowering the concentrations of several compounds related to the dihydroartemisinic (DHAA) branch of the biosynthetic pathway of AN. Response waves were similar among treatments, and only the timings varied. Results demonstrated that the applied stress initiated transient response waves through the DHAA related branch of biosynthetic pathway of AN. The speed of responses varied, with treatment of salicylic acid and chitosan oligosaccharide producing the fastest responses, followed by H2O2, NaCl and sandblasting.