Nanocurcumin Innovation as an Anti-Apoptosis of Ovarian Granulosa Cells in White Rats Exposed to Lead Acetate (PbAc)

Anis Satus Syarifah
2022 Babali Nursing Research  
Exposure to Pb causes increased apoptosis of ovarian granulosa cells through oxidative stress mechanism. Curcumin has protective effects on reproductive organs, anti-apoptotic, antioxidant in normal cells. Curcumin in innovated nano form can function as an effective anti-apoptosis in ovarian granulosa cells of rats due to PbAc exposure. Methods: Thirty female rats were divided into 3 groups, the negative control group (the rats receiving distilled water, in each 90 minutes receiving corn oil),
more » ... ositive control group (the rats receiving PbAc of 30 mg/kg BW, in each 90 minutes receiving corn oil), the experimental group, in which the rats receiving PbAc of 30 mg/kg BW, and in each 90 minutes receiving nanocurcumin of 200 mg/kg BW. All groups received treatment orally once a day for 30 days. On day 31 the rats to granulosa cell apoptosis examination using Tunnel method. Results: Rate of apoptosis was in the positive control group (5.4 ± 0.8%/micro) and the lowest was in the experimental group (1.1 ± 0.5%/micro) and the negative control group (1.2 ± 0.6). The experimental group showed the same p value as the negative control group (p = .095) and different p value (p = .010) from the positive control group. These findings indicated that the innovation of curcumin in nano form at a dose of 200 mg/KgBW reduced apoptosis of rat ovarian granulosa cells due to PbAc exposure. Conclusion: The innovation of curcumin in nano form has the potential as an effective natural anti-apoptosis in rats ovarian granulosa cells exposed to PbAc.
doi:10.37363/bnr.2022.3279 fatcat:pttgogy5ifaormf7245rbrm3iy