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The missing nucleoside experiment: a new technique to study recognition of DNA by protein
W e report a new technique for quickly determining which nucleosides in a D N A molecule are contacted by a sequence-specific DNA-binding protein. Our method is related to the recently reported "missing contact" experiment [Brunelle, A., & Schleif, R. F. (1987) Proc. Nutl. Acud. Sci. U.S.A. 84,doi:10.1021/bi00450a041 pmid:2611245 fatcat:n5nzxyjgivc6vp275e5dutuube