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Vitamin D was separated from the rachitogenic substance in dry grass and hay by chromatography [no details]. Fractions of the unsaponifiable residue of the grass fat, with addition of fixed amounts of vitamin D, were tested on rachitic rats, with radiography of the bones as criterion, to ascertain the content of rachitogenic substance. A fraction was obtained which contained all the vitamin D but not the rachitogenic substance. The last was present chiefly in the carotene fraction; the fractiondoi:10.18174/njas.v2i1.17855 fatcat:u2m2ayfn7jfetkezoukexzagei