This is the first study to evaluate the efficacy of an autodissemination approach as suggested by WHO. Therefore, efficacy of an autodissemination approach in small-scale field trials against wild Aedes sp. population was evaluated in an urbanised setting, Malaysia. Lethal ovitraps enhanced with pyriproxyfen were used to control Aedes sp. populations at treatment sites, with the autodissemination activity was evaluated using the WHO larva bioassays. Lethal ovitraps enhanced with pyriproxyfen

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... ectively reduced of Aedes sp. population. All autodissemination stations were shown to be visited by Aedes sp. mosquitoes with 100% complete inhibition against eggs and larvae development. In the larvae bioassay, pupae mortality ranged from 14 to 40%. Statistically a significant reduction of Aedes sp. population in the treatment sites compared to the untreated areas. The study proved for the autodissemination of pyriproxyfen to breeding habitats by wild Aedes sp. This technique is highly potentially for vector control activities. Future evaluation should focus on large-scale field trials.