Receptor Tyrosine Kinase Targeting in Multicellular Spheroids [chapter]

Susan Breslin, Lorraine O'Driscoll
2014 Msphere  
While growing cells as a monolayer is the traditional method for cell culture, the incorporation of multicellular spheroids into experimental design is becoming increasingly popular. This is due to the understanding that cells grown as spheroids tend to replicate the in vivo situation more reliably than monolayer cells. Thus, the use of multicellular spheroids may be more clinically relevant than monolayer cell cultures. Here, we describe methods for multicellular 3D spheroid generation that
more » ... be used to provide samples for receptor tyrosine kinase (and other protein) detection. Methods described include the forced-fl oating poly-HEMA method, the hanging-drop method, and the use of ECM to form multicellular 3D spheroids.
doi:10.1007/978-1-4939-1789-1_15 pmid:25319898 fatcat:wvrvkubj6zblrdaui4v4r2ztma