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Identification and Characterization of Single Strand DNA-Binding Protein That Represses Growth Hormone Receptor Gene Expression
The GH receptor is essential for the actions of GH on growth and metabolism. Electromobility shift assay established that a 42-bp enhancer element in the promoter of the L1 transcript of the murine GH receptor bound nuclear proteins specific for the coding strand or the DNA duplex. Using methylation interference footprinting and electromobility shift assay with mutant oligonucleotides, the DNA-binding sites for the single-strand DNA-binding protein (SSBP) and the double-strand DNAbindingdoi:10.1210/mend.11.9.9967 pmid:9259320 fatcat:33uf5g32orgxxblqnipjxuy6iu