Historically, recombinant membrane protein production has been a major challenge meaning that many fewer membrane protein structures have been published than those of soluble proteins. However, there has been a recent, almost exponential increase in the number of membrane protein structures being deposited in the Protein Data Bank. This suggests that empirical methods are now available that can ensure the required protein supply for these difficult targets. This review focuses on methods that

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... e available for protein production in yeast, which is an important source of recombinant eukaryotic membrane proteins. We provide an overview of approaches to optimize the expression plasmid, host cell and culture conditions, as well as the extraction and purification of functional protein for crystallization trials in preparation for structural studies. fluorescent protein; GPCR, G protein-coupled receptor; HSR, heat shock response; MNG, maltose neopentyl glycol; ORF, open reading frame; SEM, standard error of the mean; SMA, styrene maleic acid; SMALP, SMA lipid particles; T4L, T4 lyzozyme; UPR, unfolded protein response; v/v, percent by volume of solute in the total volume of solution. Methods 95 (2016) 26-37