Metabolic Engineering ofCorynebacterium glutamicumfor Cadaverine Fermentation

Takashi MIMITSUKA, Hideki SAWAI, Masahiro HATSU, Katsushige YAMADA
2007 Bioscience, biotechnology and biochemistry  
Cadaverine, the expected raw material of polyamides, is produced by decarboxylation of L-lysine. If we could produce cadaverine from the cheapest sugar, and as a renewable resource, it would be an effective solution against global warming, but there has been no attempt to produce cadaverine from glucose by fermentation. We focused on Corynebacterium glutamicum, whose Llysine fermentation ability is superior, and constructed a metabolically engineered C. glutamicum in which the L-homoserine
more » ... e L-homoserine dehydrogenase gene (hom) was replaced by the L-lysine decarboxylase gene (cadA) of Escherichia coli. In this recombinant strain, cadaverine was produced at a concentration of 2.6 g/l, equivalent to up to 9.1% (molecular yield) of the glucose transformed into cadaverine in neutralizing cultivation. This is the first report of cadaverine fermentation by C. glutamicum.
doi:10.1271/bbb.60699 pmid:17895539 fatcat:rxcoeknymvfcnjf7u2rotbsvxa