PURIFICATION OF TYPE B STAPHYLOCOCCAL ENTEROTOXIN1

James I. Frea, Elizabeth McCoy, F. M. Strong
1963 Journal of Bacteriology  
FREA, JAMES I. (University of Wisconsin, Madison), ELIZABETH MCCOY, AND F. M. STRONG. Purification of type B staphylococcal enterotoxin. J. Bacteriol. 86:1308Bacteriol. 86: -1313Bacteriol. 86: . 1963.-A procedure for the production and isolation of type B staphylococcal enterotoxin in yields up to 4 mg per liter of toxic culture is presented. Staphylococcus aureus S6 was grown in shake flask cultures at 37 C for 24 hr in a medium consisting of 2% Hy-Case S.F., 0.3 % yeast extract, calcium
more » ... ract, calcium pantothenate, nicotinic acid, and thiamine. Cells were removed by centrifugation and the supernatant fluid was concentrated by dialysis against polyethylene glycol. Enterotoxin was isolated from the concentrate by ethanol precipitation, gel filtration on a G-100 Sephadex column, and Sephadexcolumn electrophoresis. The purified toxin was identical to type B reference enterotoxin on both disc electrophoresis and Ouchterlony gel diffusion.
doi:10.1128/jb.86.6.1308-1313.1963 fatcat:45epd2r4wfc5lk5ufiq5sljn2y