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Original Article Establishment of human endometriosis-derived immortalized eutopic endometrium stromal and epithelial cell lines
Int J Clin Exp Med
Endometriosis (EM) is commonly seen in women at reproductive age and can have a significant social and psychological impact. However, reliable, simple in vitro models of endometriosis are not common. To establish human EM-derived immortalized cell lines, we transfected cells from eutopic and ectopic endometria of endometriosis patients with the plasmid PCD2SV40T. The cells were then screened with G418, and monoclonal cells were isolated by the limited dilution method. Subclones were evaluatedfatcat:5til2uvclzdwdj44nzrl6w743u