MOESM1 of Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin

Micheline Guillotte, Farida Nato, Alexandre Juillerat, Audrey Hessel, Françoise Marchand, Anita Lewit-Bentley, Graham Bentley, Inès Vigan-Womas, Odile Mercereau-Puijalon
2016 Figshare  
Additional file 1: Figure S1. Impact of biotinylation of mAbs raised to bDBL1 on their ELISA reactivity. Dose-dependent reactivity of biotinylated mAbs assessed by ELISA on serial dilutions of bDBL1 antigen. ELISA plates with coated with decreasing concentrations of bDBL1 (threefold dilution series in PBS buffer), and processed as described in the "Methods" section. Pairs of biotinylated and native mAbs were tested in parallel at a dilution of 20 ng mL−1.
doi:10.6084/m9.figshare.c.3645116_d3 fatcat:akyjxoa7y5aqzmrhy5uxcbzkoq